Photochemistry and UV fluorescence
Below the visible edge. Used for photo-initiated chemistry, resist exposure and UV-excited fluorophores.
Lambda Beam 532 nm DPSS 100mW - CW compact laser module
USD $6,214.00
Description
DPSS CW Laser
All inclusive, plug-and-play. The Lambda Beam CW DPSS laser is a high-performance, modulatable laser source designed for seamless integration. This German-engineered, all-inclusive diode-pumped solid-state laser system boasts a compact footprint without compromising on power or precision. Renowned for its remarkable durability and excellent beam quality, the Lambda Beam DPSS laser offers unparalleled versatility. With various options for system integration, it provides the highest flexibility for demanding industrial, scientific, and and medical applications, making it a truly universal solution
Ideal for
Software Compatibility
Specifications
Shipping Information
Return Policy
Interactive selection tool
Plan your 532 nm setup
Three views built from this laser's published specifications: where 532 nm sits in the Lambda Beam range, how the beam grows across your bench, and which configuration suits your application.
The Lambda Beam range, plotted on a real spectral axis
Every tick sits at its true position between 375 and 800 nm. Select a line to see what it is used for.
375 nmvisible800 nm
DAPI, Hoechst and flow cytometry
The workhorse violet line for nuclear stains, Brilliant Violet dyes, forensics and direct-write lithography.
Illumination and projection
A high-power direct blue diode line, common in projection, illumination and broad fluorescence excitation.
Optogenetics and HeCd replacement
Matches channelrhodopsin-2 activation and stands in for the 442 nm helium-cadmium line.
GFP, FITC and flow cytometry
The argon-ion replacement line. Still the default excitation for GFP, FITC and Alexa 488 in confocal and cytometry.
YFP and holography
Sits between the blue and green bands for YFP excitation and interference work where 532 nm is too close to a dye peak.
This laser: Raman, confocal, holography, LIDAR
The most widely deployed green line. Frequency-doubled Nd:YVO4 output with TEM00 quality, chosen for Raman, confocal microscopy, holography, metrology, speckle interferometry and as a HeNe replacement.
mCherry, DsRed and RFP excitation
The yellow-green line that excites red fluorescent proteins far more efficiently than 532 nm.
APC, Cy5 and machine vision
Red excitation for far-red dyes, plus alignment, machine vision and Raman on strongly fluorescing samples.
Photobiology and PDT research
Deep red output used in photodynamic therapy research and photobiology, where tissue penetration improves with wavelength.
Raman with fluorescence suppression
Invisible to the eye. The standard Raman line when 532 nm excitation buries the spectrum in sample fluorescence. Available with the Wavelock option for a sub-0.015 nm stabilised linewidth.
Output power options and controller choice vary by wavelength. Download the full Lambda Beam datasheet or browse the CW laser modules in stock.
How big is the spot on your bench?
Computed from the published aperture diameter of 1.2 mm and a full-angle divergence below 1.2 mrad. Choose a working distance.
Aperture
1.2 mm
1.2 mm
Transverse scale is 1 mm to 7 px. Propagation distance is not to scale. Irradiance is averaged across the beam area.
Distance0.25 m
Beam diameter1.50mm
Irradiance, 100 mW5.66 W/cm²
Irradiance, 200 mW11.32 W/cm²
Distance0.5 m
Beam diameter1.80mm
Irradiance, 100 mW3.93 W/cm²
Irradiance, 200 mW7.86 W/cm²
Distance1 m
Beam diameter2.40mm
Irradiance, 100 mW2.21 W/cm²
Irradiance, 200 mW4.42 W/cm²
Distance2 m
Beam diameter3.60mm
Irradiance, 100 mW982 mW/cm²
Irradiance, 200 mW1.96 W/cm²
Distance5 m
Beam diameter7.20mm
Irradiance, 100 mW246 mW/cm²
Irradiance, 200 mW491 mW/cm²
Distance10 m
Beam diameter13.20mm
Irradiance, 100 mW73 mW/cm²
Irradiance, 200 mW146 mW/cm²
Straight-line far-field estimate for planning only. The on-axis peak of a Gaussian beam is about twice the average figure shown. A 532 nm continuous-wave source at these powers is a Class 3B laser: enclose the beam path and wear rated eyewear. Ask Axiom Optics about beam expanders and fibre delivery.
Which configuration fits your application?
Select what you are building. Each answer names the output power to order, what to specify with it, and the one thing worth checking first.
Why 532 nm. 532 nm gives roughly four times the Raman scattering efficiency of 785 nm, so weak inorganic, carbon and semiconductor bands come up fast at modest integration times.
Specify with it
- Full 200 mW so you can attenuate down to a damage-safe power and keep headroom
- Active power control mode for repeatable band intensities between sessions
- A clean-up filter, since a DPSS line still carries pump leakage
Fluorescent samples. Biological and polymer samples usually swamp a 532 nm spectrum with fluorescence. Move to the 785 nm Wavelock for those.
Why 532 nm. 532 nm excites rhodamine, Alexa 532, tdTomato and similar orange-emitting labels near their peak, and the TEM00 profile fills a high-NA objective pupil cleanly.
Specify with it
- 100 mW is generous at the sample; scan powers are typically microwatts
- The fiber coupler to keep the head off the microscope frame and deliver a clean point source
- Modulation input wired to your scan controller for flyback blanking
Red fluorescent proteins. mCherry and DsRed excite far more efficiently at 561 nm. Ask about a 561 nm head if RFPs are your main label.
Why 532 nm. A stable single transverse mode and low pointing drift are what fringe contrast depends on, and 532 nm keeps the fringe pitch comfortable for standard sensors.
Specify with it
- 100 mW is normally ample once the beam is expanded
- Temperature stabilisation left on and a warm-up period before capture
- Active power control mode to hold fringe brightness across an exposure series
Path difference. A standard DPSS head is not single-frequency, so contrast falls away once the two arms differ by more than a few centimetres. For long unequal paths, ask about the Wavelock single-frequency option.
Why 532 nm. A green HeNe delivers a few milliwatts from a metre-long tube. This head delivers 100 mW from a package the size of a deck of cards, with no plasma tube to age out.
Specify with it
- 100 mW, then attenuate to match your existing HeNe budget
- PowerBox controller if the head is going straight into an existing enclosure
- Ltune over USB to log operating hours and set a fixed output power
Wavelength shift. A HeNe green line sits at 543.5 nm, not 532 nm. Check any dichroic, notch or edge filter in the path before you swap.
Why 532 nm. Visible green returns well from vegetation, water and painted surfaces, and the modulation input lets you gate or encode the outgoing beam directly.
Specify with it
- Full 200 mW to hold signal-to-noise at range
- Modulation input driven from your timing electronics
- A beam expander if you need to hold the spot down past 10 m
Eye safety at range. A collimated green beam stays hazardous well beyond the working area. Terminate the beam path and control access.
Why 532 nm. Round 1.2 mm TEM00 output with under 1.2 mrad divergence gives a centroid a position sensor can trust, and under 3 percent drift over eight hours keeps a calibration honest.
Specify with it
- 100 mW, usually attenuated hard at the detector
- Active power control mode so detector response is not tracking laser drift
- Rigid kinematic mounting; pointing stability is set by your mount, not the head
Thermal settling. Give the head its full warm-up before you take reference measurements, or the first hour of data will carry a drift term.
Why 532 nm. Illuminating a full field of view or a light sheet spends power quickly, and speckle contrast depends on the beam staying spatially coherent across the whole illuminated area.
Specify with it
- Full 200 mW to fill a field of view or form a light sheet
- Sheet-forming or expansion optics sized to your field
- Camera exposure synchronised through the modulation input
Continuous wave, not pulsed. A CW source freezes motion only as well as your camera exposure allows. Fast flows still need a pulsed system.
Why 532 nm. Stable, dose-repeatable output is the whole requirement in a light-dose study, and active power control holds the delivered irradiance flat across a long exposure.
Specify with it
- Full 200 mW so fibre and coupling losses still leave you the dose you need
- The fiber coupler for delivery to the sample stage or probe
- Ltune logging of operating hours and set power for your study record
Photosensitiser match. 532 nm suits Rose Bengal and similar. Most clinical photosensitisers absorb in the deep red, closer to 660 nm.
Not seeing your application? Axiom Optics supplies the full RGB Lasersystems range from 375 to 1550 nm. Tell us the experiment and we will spec the head.
